| Subject 1. Historical introduction. Principles of Immunochemistry. |
1.1. Discovery and identification of molecular components such as antibodies, receptors and antigen.
1.2. Development of techniques such as agglutination/precipitation, neutralization, lysis by the complement system, which allowed the characterization and understanding of the immunological reaction.
1.3. The relevance of transplants and allergy for the development of Immunology.
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| Subject 2. Components of the immune system. Basic concepts. |
2.1. Membrane receptors and soluble molecules.
2.2. Cells. |
| Subject 4. Basic concepts of Immunochemistry and Immunogenetics |
4.1. Antibodies.
4.2. Receptors involved in the immune response.
4.3. Concept of antigen, hapten and immunogen.
4.4. Antibody-antigen and TCR-peptide-MHC interaction.
4.5. Principles of the genetic diversity of receptors. |
| Subject 6. Immunochemistry tecniques |
6.1. Homogeneous techniques.
•Precipitation
•Agglutination
•Complement. Quantification of
components
6.1. Heterogeneous techniques.
•Principles of colorimetry, fluorescence,
chemiluminescence and radioactivity.
•Visualization techniques: optics, fluorescence,
electronic, confocal
•ELISA: direct, indirect, competitive , sandwich
•EIA, RIA
•Western Blot and Dot Blot
•Immunoprecipitation
•Immunofluorescence
•Enzymatic techniques: Immunohistochemistry /
Immunocytochemistry |
| Laboratory practices |
1) Agglutination technique
2) Antigen-antibody conjugation
3) ELISA
4) Dot Blot
5) Cell separation by density gradient centrifugation |
