Guia docente 2023_24 Facultade de Química

Contents

Topic	Sub-topic
Subject 1. Historical introduction. Bases of the *Inmunoquímica	1.1. Discovery and identification of molecular components like Antibodies, Immune answer and Antígeno. 1.2. Development of technicians like the agglutination/precipitation, neutralisation, lisis by complement, that allowed his characterisation and understand the immunological reaction. 1.3. The importance of the *transplantes and allergy for the development of the Immunology.
Subject 2. Components Immune system. Basic concepts.	2.1. Receptors of membrane and soluble Molecules. 2.2. Cells.
Subject 3. Introduction to the Cellular Immunology	3.1. Main cellular types of the IF and his function. 3.2. Concept of phenotype and cellular differentiation. 3.3. The *CDs like markers of cellular differentiation.
Subject 4. Basic concepts of Inmunoquímica and Inmunogenética	4.1. The antibodies. 4.2. The TCR and the MHC. 4.3. Concept of antígeno, hapteno and inmunógeno. 4.4. Interaction antígeno - antibody and TCR-peptide-MHC. 4.5. Genetic bases of the diversity of receptors.
Subject 5. Components of the *Inmunoensayos	5.1. Obtaining of antibodies in the laboratory 5.2. Technicians of purification and *escalado 5.3. Chemical modification of the antibodies
Subject 6. Technical *Inmunoquímicas	6.1. Technical homogéneas. •Technicians of Precipitation. •Agglutination. •I complement. Quantification of his components. 6.1. Heterogeneous technicians. •Principles of colorimetry, fluorescence, chemiluminescence and radioactividad •Technical of visualisation: optics, fluorescent, electronic, confocal •ELISA: direct, indirect, competitive , sándwich •EIA, RIA •Inmunodetección by Western Blot and Dot Blot •Inmunoprecipitación •Technical of Inmunofluorescencia •Technical enzymatic: Inmunohistoquímica / Inmunocitoquímica
Subject 7. *Inmunoensayos In the pharmaceutical industry	7.1. Importance in the development of medicines and clinical appearances
Practices of laboratory	1) Technical of Agglutination 2) Conjugation antígeno / antibody 3) ELISA 4) Dot *blot 5) Separation of cells by gradient of density