Guia docente 2023_24 Facultade de Ciencias do Mar

Contents

Topic	Sub-topic
Lesson 1. Microorganisms on the marine ambient	1.1. Purpose and field of study of marine microbiology 1.2. Microorganisms on the biological scale. 1.3. Role of microbiota in marine ecosystems. 1.4. Perspectives of marine microbiology
Lesson 2. Structure and function of prokaryotic microorganisms and acellular agents	2.1. Structure and function of prokaryotic microorganisms 2.2. Structure and function of acellular agents
Lesson 3. Microbial phisiology	3.1. Microbial growth in the laboratory: mathematical expression 3.2. Microbial growth in the marine environment: effect of environmental factors 3.3. Cooperative and multicellular processes 3.4. Asexual reproduction in bacteria
Lesson 4.- Methods of study of marine microbiota: culture-dependent techniques	4.1. Concepts of asepsis and sterilisation 4.2. Sampling techniques. 4.3. Isolation, cultivation and conservation techniques .4.4. Techniques of cuantification. 4.5. Techniques for the characterisation of pure cultures.
Lesson 5.- Methods of study of marine microbiota: non-cultivation dependent techniques	5.1. U.V. light microscopy: unespeciphic fluorescence . 5.2. Flow Cytometry 5.3. In Situ Hybridization Techniques 5.4. Selective Amplification and sequencing: PCR; DGGE; NGS sequencing techniques 5.5. Principles of Metagenomic Analysis
Lessons 6. Diversity of marine microbiota.	6.1. Relevant species in the Bacteria, Archaea and Eucarya domains. Position in the phylogenetic tree. 6.2. Microorganisms in the trophic chain. 6.3. Microorganisms in the element cycles 6.4 Symbiotic associations with animals and plants 6.5. Diversity of Viruses and Bacteriophages Role in marine microbial ecosystems
LABORATORY PRACTICAL	1. Preparation of culture media 2. Sampling of environmental samples 3. Isolation and preservation of pure cultures 4. Cuantification of microorganisms 5. Tests of bacterial identification.