Guia docente 2023_24 Facultade de Química

Contents

Topic	Sub-topic
Topic 1. Immunoassay	Introduction. In vitro antigen-antibody reaction. Immunoassay techniques without marker. Immunoassay techniques with marker: generalities. Radioimmunoassay. Enzyme immunoassay. Fluoroimmunoassay. Luminoimmunoassay.
Topic 2. Enzymatic methods of analysis	Introduction. Enzymatic end-point methods: single-step methods and methods with coupled reactions. Enzyme kinetic methods: methods based on zero-order kinetics and methods based on one-order kinetics.
Topic 3. Determination of nucleic acids: Hybridization and PCR techniques	Introduction. Nucleic acid extraction and purification techniques. Hybridization assays: liquid phase, solid phase and in situ. Polymerase chain reaction: basics. Variants of classical PCR.
Topic 4. Automation and miniaturization	Introduction. Automation: generalities. Analyzers. Flow Injection Analysis (FIA). Sequential injection analysis (SIA). Miniaturization: fundamentals and approaches.
Topic 5. Chemical sensors and biosensors	Introduction. Recognition systems. Classification of chemical sensors and biosensors. Analytical characteristics of the sensors. Applications of interest.
Topic 6. Chemometrics	Introduction to chemometrics. Structure of hypothesis testing. Rejection of anomalous results. Comparison of analytical results: parametric and non-parametric tests. Control charts. Introduction to experimental design.