Guia docente 2020_21 Facultade de Química

Contents

Topic	Sub-topic
1.Biomolecules	Structure and structure-function relationship of biomolecules: proteins, carbohydrates, lipids and nucleic acids.
2.Biocatalisis	Structure and function of enzymes. Enzymatic reactions. Enzymatic kinetics.
3.Vitamins and coenzymes	Structure and function of vitamins and coenzymes in metabolic reactions.
4.Metabolism of glucides	Degradative Metabolism of glucides: glycolysis. Metabolic crossroad of pyruvate. Degradative Oxidation of acetil-CoA. Respiratory chain and oxidative phosphorylation. Oxidative Route of the pentoses phosphate. Gluconeogénesis. Metabolism of glycogen.
5. Metabolism of lipids	Degradation of lipids: oxidation of fatty acids. Biosynthesis of fatty acids.
6. Metabolism of proteins	Proteolisis. Degradation of amino acids. Destination of the ion ammonium. Biosynthesis of amino acids.
7.Metabolism of nucleotides	Degradation of nucleic acids and nucleotides. Biosynthesis of nucleotides.
8.Experimental methods in Biochemistry	Techniques for synthesis and isolation of biomolecules. Separation, determination and identification of proteins. Determination and quantification of lipids. Determination and quantification of glycogen. Evaluation of the enzymatic activity. Effect of the temperature and inhibition. Polymerase chain reaction. Use of restriction enzymes.