Guia docente 2017_18 Facultade de Química

Contents

Topic	Sub-topic
1.Biomolecules	Carbohydrates: Classification and structure. Lipids: Classification and structure. Biological functions of the lipids. Proteins: Structure and configuration of the proteins. Relation structure -function. Nucleic Acids: Structure and function.
2.Biocatalisis	Nomenclature and classification of the enzymes Enzymatic Kinetics Mechanisms of the enzymatic reactions Effect of the temperature in the enzymatic reaction and inhibition Quantification of the enzimatic activity. Alosteric enzymes
3.Vitamins and coenzymes	Structure and role in metabolic reactions
4.Metabolism of glucides	Degradative Metabolism of glucides: glycolysis. Metabolic crossroad of pyruvate. Degradative Oxidation of acetil-CoA. Respiratory chain and oxidative phosphorylation. Oxidative Route of the pentoses phosphate. Gluconeogénesis. Metabolism of glycogen.
5. Metabolism of lipids	Degradation of lipids: oxidation of fatty acids . Biosynthesis of fatty acids.
6. Metabolism of proteins	Proteolisis. Degradation of amino acids. Destination of the ion ammonium. Biosynthesis of amino acids.
7.Metabolism of nucleotides	Degradation of nucleic acids and nucleotides. Biosynthesis of nucleotides.
8.Experimental methods in Biochemistry	Technics for synthesis and isolation of biomolécules Separation, determination and identification of proteins Determination and quantification of lipids Determination and quantification of glycogen Evaluation of the enzymatic activity. Effect of the temperature and inhibition Polymerase chain reaction. Utilisation of restriction enzymes