Guia docente 2023_24 Facultade de Bioloxía

Contents

Topic	Sub-topic
Lesson 1. Unix for Bioinformatics	Unix environment and command line. Remote servers. File access and manipulation. Regular expressions. Bash utilities and scripts.
Lesson 2. High-throughput DNA sequencing.	Sequencing platforms. Sequencing libraries. Sequencing coverage. FASTQ format. Read quality control.
Lesson 3. Sequence alignment	Concept of alignment. Scoring matrices. Alignment algorithms. Sequencing read mapping. SAM/BAM formats Post-processing.
Lesson 4. Variant calling	Types of variants. Identification. VCF format. Variant filtering. Annotation. Somatic variants.
Lesson 5. Quantification of gene expression	RNA-seq. Experimental design. RNA-seq alignment. Quantification. Differential expression analysis.
Lesson 6. Metagenomic analysis	Microbiome. Metagenomics. 16S analysis. Shotgun analysis. Alpha and beta diversity. Metagenomic annotation.
Lesson 7. Genome assembly and annotation	Assembly. Contigs and scaffolds. K-mer analysis. Assembly quality control. Genome annotation.