Guia docente 2023_24 Facultade de Bioloxía

Contents

Topic	Sub-topic
1. Cell signalization.	Signaling systems. Intracellular receptors. Membrane receptors. Tyrosine kinase receptors . Receptors of cytokines. Receptors linked to protein G. Signaling routes.
2. Hormonal regulation.	Hormone regulation of metabolism. Main hormones involved in metabolism regulation.
3. Regulation of glycogen metabolism.	Regulation of glycogen degradation and synthesis: glycogen phosphorylase and glycogen synthase. Hormonal regulation of glycogen metabolism in muscle and liver.
4. Regulation of glucose metabolism.	Incorporation of carbohydrates from the diet to the glucidic metabolism. Uptake of glucose by tissues. Regulation of glycolysis. Regulation of gluconeogenesis. Regulation of the pentose phosphate route.
5. Regulation of lipidic metabolism.	Incorporation of lipids from the diet to lipid metabolism. Lipid transport: lipoproteins. Regulation of cholesterol synthesis and degradation. Regulation of the synthesis and degradation of triacylglycerols and fatty acids.
Integration and metabolism central route regulation	Regulation of the enzymatic complex pyruvate dehydrogenase. Regulation of the respiratory chain and oxidative phosphorylation.
Metabolic specialization of the main organs	Metabolic interrelations in various nutritional states. Metabolic specialization of the organs.
Metabolism of proteins.	Protein destinations. Degradation of proteins. Ubiquitin and proteasome. Metabolism of ammonium ion.
Practice 1	Determination of the activity of the enzyme pyruvate kinase.
Practice 2	Determination of the activity of the enzyme succinate dehydrogenase
Practice 3	Kinetics of a metabolic enzyme
Practice 4	Respiratory chain and oxidative phosphorylation. Theoretical experiments
Practice 5	Determination of the activity of the alkaline phosphatase enzyme.
Practice 6	Isolation of glycogen from liver and kidney
Practice 7	Quantification of glycogen concentration