This matter pretends to give a wide but concise coverage to the technicians of DNA *recombinante. It is thought for graduated, researchers of other fields that wish to enter in these procedures and professionals of the sector *biotecnológico. The matter begins with an introduction of the basic biochemical principles in which it bases this technology. They describe to continuation the reaction in chain of the polymerase and the molecular cloning using to the bacterium *Escherichia *coli like *hospedador and describing his *plásmidos, *fagos and hybrid vectors associated. Next it tackles the construction and track of *genotecas and like modifying, *inactivar or express sequences cloned. Finally, it argues the genetic manipulation in other organisms. Besides, they make some practical sessions in which they carry out distinct procedures of cloning and expression of genes that allow to the students contrast his knowledges and be evaluated of way more complete.
