Guia docente 2024_25 Facultad de Biología

Contents

Topic	Sub-topic
Lesson 1. Unix for Bioinformatics	Unix environment and command line. Remote servers. File access and manipulation. Regular expressions. Bash utilities and scripts.
Lesson 2. High-throughput DNA sequencing.	Sequencing platforms. Sequencing libraries. Sequencing coverage. FASTQ format. Read quality control.
Lesson 3. Sequence alignment	Alignment. Scoring. Alignment algorithms. Sequencing read mapping. SAM/BAM formats Post-processing.
Lesson 4. Variant calling	Types of variants. Identification. Calling strategies. VCF format. Structural variation. Filtering. Annotation
Lesson 5. Quantification of gene expression	RNA-seq. Experimental design. RNA-seq alignment. Quantification. Differential expression analysis.
Lesson 6. Genome assembly and annotation	Assembly. Algorithms. Evaluation. K-mer analysis. Genome annotation.
Lesson 7. Metagenomic analysis	Microbiome. Metagenomics. 16S analysis. Shotgun analysis. Alpha and beta diversity. Metagenomic annotation