Subject 1. Historical introduction. Bases of the *Inmunoquímica |
1.1. Discovery and identification of molecular components like Antibodies, Immune answer and *Antígeno.
1.2. Development of technicians like the agglutination/precipitation, neutralisation, *lisis by complement, that allowed his characterisation and understand the immunological reaction.
1.3. The importance of the *transplantes and allergy for the development of the Immunology. |
Subject 2. Components Immune system. Basic concepts. |
2.1. Receptors of membrane and soluble Molecules.
2.2. Cells. |
Subject 4. Basic concepts of *Inmunoquímica and *Inmunogenética |
4.1. The antibodies.
4.2. The *TCR and the *MHC.
4.3. Concept of *antígeno, *hapteno and *inmunógeno.
4.4. Interaction *antígeno - antibody and *TCR-peptide-*MHC.
4.5. Genetic bases of the diversity of receptors. |
Subject 6. Technical *Inmunoquímicas |
6.1. Technical *homogéneas.
•Technicians of Precipitation.
•Agglutination.
•I complement. Quantification of his
components.
6.1. Heterogeneous technicians.
•Principles of colorimetry, fluorescence,
chemiluminescence and *radioactividad
•Technical of visualisation: optics, fluorescent,
electronic, *confocal
•ELISA: direct, indirect, competitive , *sándwich
•*EIA, RIA
•*Inmunodetección by *Western *Blot and *Dot *Blot
•*Inmunoprecipitación
•Technical of *Inmunofluorescencia
•Technical enzymatic: *Inmunohistoquímica /
*Inmunocitoquímica |
Practices of laboratory |
1) Technical of Agglutination
2) Conjugation *antígeno / antibody
3) ELISA
4) *Dot *blot
5) Separation of cells by gradient of density |