2.Biocatalisis |
Structure and function of enzymes.
Enzymatic reactions.
Enzymatic kinetics.
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4.Metabolism of glucides |
Degradative Metabolism of glucides: glycolysis.
Metabolic crossroad of pyruvate. Degradative Oxidation of acetil-CoA.
Respiratory chain and oxidative phosphorylation. Oxidative Route of the pentoses phosphate. Gluconeogénesis. Metabolism of glycogen. |
6. Metabolism of proteins |
Proteolisis.
Degradation of amino acids.
Destination of the ion ammonium.
Biosynthesis of amino acids. |
8.Experimental methods in Biochemistry |
Techniques for synthesis and isolation of biomolecules.
Separation, determination and identification of proteins.
Determination and quantification of lipids.
Determination and quantification of glycogen.
Evaluation of the enzymatic activity. Effect of the temperature and inhibition.
Polymerase chain reaction.
Use of restriction enzymes. |