2.Biocatalisis |
Nomenclature and classification of the enzymes
Enzymatic Kinetics
Mechanisms of the enzymatic reactions
Effect of the temperature in the
enzymatic reaction and inhibition
Quantification of the enzimatic activity.
Alosteric enzymes |
4.Metabolism of glucides |
Degradative Metabolism of glucides: glycolysis.
Metabolic crossroad of pyruvate. Degradative Oxidation of acetil-CoA.
Respiratory chain and oxidative phosphorylation. Oxidative Route of the pentoses phosphate. Gluconeogénesis. Metabolism of glycogen. |
6. Metabolism of proteins |
Proteolisis.
Degradation of amino acids.
Destination of the ion ammonium.
Biosynthesis of amino acids. |
8.Experimental methods in Biochemistry |
Technics for synthesis and isolation of biomolécules
Separation, determination and identification of proteins
Determination and quantification of lipids
Determination and quantification of glycogen
Evaluation of the enzymatic activity. Effect of the temperature and inhibition
Polymerase chain reaction.
Utilisation of restriction enzymes |